Anti-PCNA Antibody

Raised in rabbit and affinity-purified for routine laboratory use, this unconjugated polyclonal reagent from Antibodies.com is supplied as a 50 µL liquid at 1 mg/mL in phosphate buffered saline (PBS) containing 0.1% sodium azide and 50% glycerol, a formulation that allows convenient dilution into standard blocking and incubation buffers. The antibody recognises proliferating cell nuclear antigen (PCNA), a highly conserved ~36 kDa nuclear scaffolding protein that loads DNA-replication and DNA-repair factors onto chromatin and serves as a widely used marker of S-phase and proliferative activity in cell-biology studies. It is validated for Western blot, immunohistochemistry, immunofluorescence and immunoprecipitation, giving laboratories a single reagent that can be deployed across biochemical, tissue-staining and co-precipitation workflows. The host species is rabbit, the clonality is polyclonal (IgG class) and the conjugate is unconjugated, so the reagent can be paired with any species-matched secondary detection system, including HRP, biotin, fluorophore or alkaline phosphatase conjugates. Reactivity has been confirmed on human, mouse and rat samples, reflecting the strong evolutionary conservation of PCNA among mammals and making the antibody useful for comparative work across the most common laboratory models. For short-term use the antibody should be kept refrigerated in its glycerol-containing buffer; for long-term storage it is typically aliquoted and held at –20 °C to preserve activity across multiple freeze-thaw cycles.