Anti-PCNA Antibody

Proliferation studies across cancer biology, regenerative medicine, and cell cycle research rely on a reliable marker of dividing cells. This unconjugated rabbit polyclonal reagent from Antibodies.com detects Proliferating Cell Nuclear Antigen (PCNA), the 36 kDa sliding clamp for DNA polymerase δ, and is supplied as 100 µl of a 1 mg/ml stock in PBS with 0.1% sodium azide and 50% glycerol.

Validated for western blot, immunohistochemistry, immunofluorescence, and immunoprecipitation, the antibody recognizes endogenous PCNA in human, mouse, and rat samples, enabling cross-species comparison of proliferation rates in tumor xenografts, regenerating tissue models, and developmental studies. In western blot, the target typically resolves as a single band near 36 kDa, while on fixed cells and tissue sections it yields a discrete nuclear staining pattern that is most intense in S-phase and other actively dividing populations.

Aliquot upon first use to limit freeze-thaw cycles and store at -20°C; the glycerol in the formulation protects the antibody during frozen storage and avoids the need for additional stabilizers. The vial format and polyclonal clonality offer broader epitope coverage than a monoclonal clone, which is helpful when surveying PCNA expression under variable fixation conditions or in tissues where antigen masking is a concern.

Common uses include confirming S-phase entry in cell cycle synchronization experiments, scoring proliferation indices on tumor microarrays, verifying successful reprogramming of induced pluripotent stem cells, and serving as a nuclear loading reference alongside lineage-specific markers in immunofluorescence panels.