Anti-PCNA Antibody

Raised in rabbit against the PCNA nuclear antigen, this polyclonal unconjugated reagent from Antibodies.com targets the 36 kDa processivity factor for DNA polymerase delta. Delivered as a 100 µl liquid formulation at 1 mg/ml in phosphate buffered saline with 0.05% sodium azide at approximately pH 7.2, the antibody is validated for western blot, immunohistochemistry, and immunocytochemistry on human, mouse, and rat samples. PCNA expression is widely used as a proliferation index in tumor biology and tissue regeneration studies, with the reagent highlighting the protein in cycling nuclei during S-phase of the cell cycle. Aliquot upon arrival to limit freeze-thaw cycles and store refrigerated at 4 °C for short-term use or at -20 °C for long-term preservation. The unconjugated format allows flexible pairing with secondary antibodies labeled with HRP, fluorophores, or biotin depending on the detection method, supporting applications such as western blot analysis of lysates from proliferating cell cultures and IHC staining of paraffin-embedded tumor sections to quantify growth fraction in research use only workflows.