Anti-PCMT1 Antibody

PCMT1 (protein-L-isoaspartate (D-aspartate) O-methyltransferase) is the enzyme responsible for repairing abnormal isoaspartyl residues that accumulate in long-lived proteins as a consequence of spontaneous asparagine deamidation and aspartate isomerization. This unconjugated rabbit polyclonal antibody from Antibodies.com recognizes PCMT1 in human and mouse samples, enabling cross-species studies of protein-repair capacity in cell lines, primary cultures, and tissue sections. It is supplied as 100 µl of polyclonal serum validated for Western blot and immunofluorescence, two readouts commonly used to confirm PCMT1 expression changes after knockdown, overexpression, or pharmacological manipulation, and to localize the enzyme within cells. Researchers studying age-related protein damage, neurodegeneration, and tumor biology rely on PCMT1 immunoreactivity to probe repair-pathway activity in their model systems. For routine handling, the reagent is best kept frozen in single-use aliquots to limit repeated freeze-thaw cycles, with brief centrifugation before staining to clear any aggregates.