Anti-PCK1 Antibody

Manufactured by Antibodies.com, this polyclonal reagent is raised in rabbit and is specifically directed against PCK1, the cytosolic phosphoenolpyruvate carboxykinase (PEPCK1) enzyme. The antibody is supplied at a concentration of 1.0 mg/mL in a 50 µl volume, formulated in phosphate-buffered saline (pH 7.4) containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol as a stabiliser; it should be stored at –20 °C with repeated freeze/thaw cycles avoided. The immunogen is a synthetic peptide derived from human PCK1, and the reagent has been affinity-purified to ensure that it recognises the endogenous levels of total PCK1 protein in validated assays. Verified applications include Western blotting (WB) and immunocytochemistry / immunofluorescence (ICC/IF), and the antibody has been confirmed to react with Human, Mouse, and Rat samples, making it a versatile choice for cross-species studies of gluconeogenic metabolism. The product is provided in an unconjugated format, allowing straightforward adaptation to a wide range of secondary-detection chemistries depending on the downstream application.