Anti-PCK1 Antibody

The polyclonal rabbit reagent supplied by Antibodies.com is raised against phosphoenolpyruvate carboxykinase 1 (PCK1), the cytosolic isoform of the rate-limiting gluconeogenic enzyme that converts oxaloacetate to phosphoenolpyruvate in hepatocytes and renal cortex. Provided as 100 µl of unconjugated antiserum, the antibody is validated for Western blot analysis and immunohistochemistry on human tissue, making it a practical tool for studies of hepatic glucose output, fasting response, and the hormonal control of gluconeogenesis by glucagon, glucocorticoids, and insulin.

Investigators studying type 2 diabetes, non-alcoholic fatty liver disease, and metabolic syndrome frequently use PCK1 staining to compare expression between control and treated cohorts, while Western blot work quantifies protein abundance across dietary, pharmacological, or genetic interventions. The rabbit host and polyclonal clonality broaden epitope coverage, supporting reliable detection of the full-length enzyme in whole-cell lysates and paraffin-embedded sections. To preserve activity, refrigerate the vial between uses; for longer projects, aliquot and freeze at -20 °C, and minimize freeze-thaw cycles by preparing single-use working stocks.