Anti-PCGF3 Antibody

Raised in rabbit against the PCGF3 target, this polyclonal reagent from Antibodies.com is supplied as an unconjugated 100 µl aliquot at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol as a stabiliser, allowing direct use in standard label-based detection workflows. PCGF3 (Polycomb Group Ring Finger 3) is a core component of the PRC1.6 variant of Polycomb Repressive Complex 1, where it partners with L3MBTL2, RING1B and MGA to catalyse monoubiquitination of histone H2A at Lys119 and to silence genes involved in lineage commitment and embryonic stem cell self-renewal.

The antibody has been validated for western blot and immunohistochemistry on mouse samples, supporting studies of chromatin-mediated transcriptional repression, Polycomb complex assembly, and developmental gene regulation. The 50% glycerol formulation permits storage at -20 °C with minimal freeze-thaw damage and shipped ready for immediate dilution into blocking buffer. Sodium azide at 0.1% acts as a microbial preservative for working stock solutions kept refrigerated.

Researchers using this PCGF3 antibody typically pair it with chromatin immunoprecipitation or co-immunoprecipitation to map PRC1.6 occupancy at target loci, and apply the unconjugated format when secondary reporter conjugates need to be chosen by the user. Mouse reactivity makes it suitable for investigation of murine ES cell differentiation and knockout phenotype studies, while the rabbit host allows dual-label experiments alongside mouse monoclonal primaries.