Anti-PCDHA13 Antibody

This polyclonal reagent from Antibodies.com targets PCDHA13 (Protocadherin Alpha 13), a calcium-dependent cell adhesion molecule of the cadherin superfamily that is predominantly expressed in the central nervous system and implicated in neuronal wiring and synaptic specification. The antibody is raised in rabbit and supplied as an unconjugated antiserum at 1 mg/ml in PBS buffer containing 0.1% sodium azide and 50% glycerol as a cryoprotectant, with a total volume of 100 µl per vial. Recommended applications include Western blot for detection of endogenous PCDHA13 in transfected cell lysates and rodent tissue homogenates, and immunohistochemistry on formaldehyde-fixed paraffin-embedded brain sections. Validated reactivity is limited to mouse and rat samples. Aliquot upon receipt and store at -20 °C, protected from light, to limit freeze-thaw cycles. Sodium azide is included as a preservative, so the reagent should not be added to live-cell preparations. Researchers commonly pair this reagent with anti-GAPDH or anti-β-tubulin loading controls when probing membrane-fraction preparations enriched for protocadherin family members.