Anti-PCAF Antibody

PCAF (P300/CBP-associated factor, also called KAT2B) is a nuclear histone acetyltransferase that acetylates lysine residues on histones H3 and H4, linking chromatin remodeling to transcriptional activation. This rabbit polyclonal reagent from Antibodies.com is raised against the PCAF target and supplied as an unconjugated, liquid-format antibody at 100 µl per vial, validated for western blot and ELISA workflows across human and mouse sample backgrounds.

The polyclonal format means recognition of multiple epitopes on PCAF, which helps when probing low-abundance target in nuclear extracts or in samples where the antigen may be partially degraded. Because the reagent is unconjugated, it can be paired with a secondary reporter of choice - HRP, fluorescent, or biotin - depending on whether the downstream read-out is chemiluminescent western blot or standard plate-based ELISA detection. Cross-reactivity data for non-listed species has not been confirmed by the manufacturer, so any use outside human and mouse reactivity should be validated empirically with positive control lysates before drawing conclusions.

Practical handling: keep the vial refrigerated and protect from light, avoid repeated freeze-thaw cycles, and briefly centrifuge before opening to recover the full volume. Aliquot upon first use if extended storage is planned. The reagent is supplied as 100 µl of liquid in a single vial and is intended for research use only.