Anti-PC Antibody

This polyclonal rabbit antibody from Antibodies.com targets pyruvate carboxylase (PC), a biotin-dependent mitochondrial enzyme that catalyzes the carboxylation of pyruvate to oxaloacetate in an ATP- and acetyl-CoA-driven reaction. PC sits at a key metabolic branch point, fueling gluconeogenesis in liver and kidney, de novo lipogenesis in adipose and mammary tissue, and glucose-stimulated insulin secretion in pancreatic beta cells, so protein-level detection is widely used to map tissue- and cell-type-specific metabolic phenotypes.

Validated for Western blot and immunohistochemistry on human and mouse samples, the reagent is supplied as an unconjugated polyclonal antibody raised in rabbit and packaged in a 50 µl vial. Research applications include profiling PC expression in metabolic-disease models such as insulin resistance, high-fat-diet-induced obesity, and non-alcoholic fatty liver disease, characterising biotinidase and holocarboxylase synthetase deficiencies in which the apo-form of PC accumulates, and exploring tumour metabolism in tissues that rewire anaplerotic flux through the enzyme. Autosomal-recessive mutations in the PC gene cause a rare inherited lacticacidaemia with psychomotor regression, and the antibody is used to assess PC abundance in patient-derived fibroblasts and matched animal model tissues by immunoblotting and IHC.

The unconjugated format lets researchers pair the reagent with their own choice of secondary antibody and detection chemistry, including fluorescent, HRP, or alkaline-phosphatase conjugates depending on whether western blot membranes or fixed tissue sections are being imaged. For short-term use, storage at refrigerated conditions (4 °C) is appropriate; aliquoting and freezing at -20 °C is recommended for longer-term storage to preserve activity across repeated freeze-thaw cycles.