Anti-Paxillin Antibody

From the Antibodies.com catalog of validated research reagents, this 50 µL aliquot of rabbit polyclonal anti-Paxillin antibody is raised in rabbit hosts and affinity-purified from rabbit antiserum by affinity chromatography using an epitope-specific immunogen. The unconjugated IgG reagent detects endogenous levels of Paxillin and is supplied at approximately 1 mg/mL in a liquid PBS formulation containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, making it suitable for sensitive Western blot (WB) and ELISA workflows. Verified reactivity covers human, mouse and rat samples, with cross-reactivity to monkey also reported on the supplier datasheet. Recommended working dilutions are typically in the 1:500–1:1000 range for WB and 1:10000 for ELISA, and the antibody should be stored at –20 °C in aliquots to preserve activity across multiple freeze/thaw cycles. As an unconjugated primary reagent, it is compatible with a wide range of HRP-, fluorophore- or biotin-conjugated secondary antibodies, supporting flexible detection in cell lysate and tissue lysate preparations.