Anti-Paxillin (Ab-88) Antibody

Offered through the Antibodies.com catalogue, this rabbit polyclonal reagent is raised against a synthetic peptide corresponding to residues86–90 (P-V-Y-G-S) of human Paxillin, allowing it to recognise the endogenous, non-phosphorylated form of the focal-adhesion protein. Supplied at 1 mg/mL in phosphate-buffered saline (without Mg²⁺ and Ca²⁺) at pH 7.4, with 150 mM NaCl, 0.02% sodium azide and 50% glycerol as stabilisers, the unconjugated IgG is affinity-purified from rabbit antiserum on an epitope-specific column and is provided in a 50 µL volume, intended for research use only. The antibody has been validated for Western blotting, where it is typically used at 1:500–1:1000, and for immunofluorescence staining (1:50–1:200), reproducing clean bands on HUVEC cell lysates and clear focal-contact staining in fixed-cell assays. Reactivity is documented for human samples, with the antibody detecting the total Paxillin pool regardless of tyrosine 88 phosphorylation state. Long-term storage at −20 °C is recommended, while the reagent remains stable at 4 °C for routine short-term use over several weeks. Available through the Antibodies.com primary antibodies range with a 50 µL pack size, this paxillin-targeting polyclonal is well suited to studies of integrin signalling, cytoskeletal remodelling and cell migration, complementing other reagents in the Paxillin product family.