Anti-Paxillin (Ab-118) Antibody

This rabbit polyclonal antibody from Antibodies.com targets paxillin at the Ab-118 epitope, a phosphorylation site at Tyrosine 118 that is regulated by focal adhesion kinase and Src family kinases during integrin engagement, cytoskeletal remodeling, and cell migration. The reagent is raised in rabbit, purified as a polyclonal preparation, and supplied unconjugated in a 100 µL vial, allowing laboratories to pair it with their preferred secondary detection chemistry or to label it directly for custom assays.

Validated for Western blot and immunohistochemistry, the antibody recognizes endogenous paxillin in human, mouse, and rat samples, supporting studies of focal adhesion turnover, mechanotransduction, and downstream signaling through FAK, CAS, and Crk-associated pathways. Researchers investigating metastatic behavior, fibroblast spreading, growth-factor-stimulated adhesion dynamics, or integrin-dependent signaling will find the unconjugated format useful for both standard chromogenic IHC on fixed tissue sections and quantitative immunoblotting of phospho-paxillin pools from cultured cells.

As a primary antibody in the Antibodies.com catalog, it fits into existing workflows using common lysis buffers supplemented with phosphatase inhibitors to preserve the labile phospho-Tyr118 modification, together with BSA or non-fat milk blocking steps chosen according to the downstream readout. Typical working dilutions cover both whole-cell lysate immunoblotting of the approximately 68 kDa paxillin band and tissue-section localization of focal adhesion structures.