Anti-PAX8 Antibody

PAX8 is a paired-box transcription factor essential for organogenesis of the thyroid gland, kidney, and Müllerian-derived tissues, and antibodies targeting this protein are widely used in surgical pathology to confirm lineage in difficult tumor cases. This unconjugated rabbit polyclonal reagent from Antibodies.com is supplied as a 100 µl aliquot and is validated for western blot (WB) and immunohistochemistry (IHC) on human and mouse samples, making it appropriate for detection in cell lysates and tissue sections from formalin-fixed paraffin-embedded specimens.

PAX8 immunoreactivity is particularly valued in diagnostic workflows that distinguish thyroid and renal cell carcinomas from morphologically similar lesions and that confirm Müllerian origin in gynecologic tumors. Because PAX8 expression is retained in carcinomas of the thyroid, kidney, and female genital tract but is typically absent in mesotheliomas and most lung adenocarcinomas, the marker is often included in antibody panels alongside calretinin, WT1, and TTF-1. The polyclonal format provides recognition of multiple epitopes, which can be advantageous when staining fixed tissue where antigenic sites may be partially masked by fixation-induced crosslinking.

For research use only, this reagent supports studies in developmental biology, oncology, and reproductive biology where PAX8-mediated transcriptional regulation is investigated. Storage refrigerated with protection from light preserves reagent activity across repeated freeze-thaw cycles, and aliquoting on first use is recommended to limit handling. Researchers evaluating lineage markers in mouse models of kidney development or human tumor tissue microarrays will find this antibody useful for highlighting PAX8-positive nuclei in normal and neoplastic epithelium.