Anti-PAX3 Antibody

A primary antibody from Antibodies.com, raised in Rabbit as a polyclonal reagent directed against PAX3. The antibody is of the IgG isotype and is supplied unconjugated, with reactivity demonstrated against Human, Mouse and Rat samples.

According to the catalogue entry, it is validated for Western Blot (WB) and Immunofluorescence (IF), while the manufacturer datasheet additionally lists IHC and ICC/IF as validated applications. Recommended dilutions are WB at 1:500–1:1,000, IHC at 1:50–1:200 and ICC/IF at 1:50–1:200. The reported molecular weight for the target is 60 kDa.

The immunogen is a recombinant fusion protein containing a sequence corresponding to amino acids 341–479 of human PAX3 (NP_852122.1). The reagent is purified by affinity chromatography, supplied as a liquid in Phosphate Buffered Saline (pH 7.3) with 50% Glycerol and 0.02% Sodium Azide, and is shipped at 4 °C for long-term storage at −20 °C, with freeze/thaw cycles to be avoided.