Anti-Pax-3 Antibody

Pax-3 (Paired box 3) is a transcription factor essential during embryonic development, particularly for neural crest cell migration and differentiation into melanocytes, glial lineages, and skeletal muscle progenitors. Mutations in PAX3 are linked to Waardenburg syndrome types 1 and 3, while aberrant Pax-3 expression is a diagnostic hallmark of alveolar rhabdomyosarcoma and a marker studied extensively in melanoma biology.

This unconjugated rabbit polyclonal antibody from Antibodies.com is raised against Pax-3 and supplied as 100 µl at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol. The glycerol-rich buffer keeps the reagent liquid at -20 °C, eliminating freeze-thaw damage and supporting long-term storage without aliquoting. The unconjugated format leaves labeling choices open, letting researchers pair it with custom fluorophores, HRP conjugates, or biotin for multiplex or amplified detection schemes.

Validated applications include Western blot (WB), immunohistochemistry (IHC), and immunofluorescence (IF). Reactivity covers Human, Mouse, and Rat Pax-3, which makes the reagent practical across standard laboratory models and clinical specimens. Because it is polyclonal, the antibody recognizes multiple epitopes on the Pax-3 protein, often producing stronger signals on partially degraded or low-abundance targets than monoclonal alternatives.

Typical uses include tracing neural crest derivatives in mouse embryo sections, profiling melanocyte differentiation, screening rhabdomyosarcoma cell lines and FFPE tumor biopsies, and studying pigment cell development. The antibody performs on both frozen sections and formalin-fixed paraffin-embedded tissues when paired with appropriate antigen retrieval.