Anti-Pax-2 Antibody

Raised in rabbit against the paired box transcription factor Pax-2, this unconjugated polyclonal reagent is supplied at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol as a 100 µl aliquot from Antibodies.com. It has been validated for western blot and immunofluorescence workflows on human, mouse, and rat samples, reflecting the strong evolutionary conservation of the Pax protein family across vertebrate species.

Pax-2 plays a central role during embryonic development of the kidney, ureter, eye, ear, and parts of the central nervous system, where it drives early patterning of the ureteric bud and regulates mesenchymal-to-epithelial transitions. In adult and tumor tissue it is most widely used as a diagnostic and research marker for renal epithelium and renal cell carcinoma, and is routinely applied to differentiate kidney-derived lesions from tumors of the adrenal gland or other abdominal sites that can otherwise look morphologically similar. Researchers working on kidney organoids, ureteric bud branching assays, or renal lineage tracing rely on anti-Pax-2 antibodies to label intermediate mesoderm derivatives and to monitor differentiation of induced pluripotent stem cells toward a nephron fate.

Because Pax-2 is a nuclear protein, the antibody produces clean nuclear staining in immunofluorescence and detects the expected band pattern in western blot. For best results, store refrigerated and protect from light to preserve the unconjugated formulation. The polyclonal format gives broader epitope coverage than a monoclonal reagent, which helps when alternative isoforms are present or when antigen retrieval in formalin-fixed tissue partially masks binding sites. Pairing it with an appropriate secondary antibody allows detection across imaging and chromogenic platforms.