Anti-PARP1 Antibody

Available through Antibodies.com, this rabbit polyclonal reagent is raised against PARP1 (Poly [ADP-ribose] polymerase 1), a 113 kDa nuclear enzyme involved in DNA damage response, chromatin remodeling, and caspase-mediated cleavage during apoptosis. The unconjugated antibody is supplied as a 50 µl liquid aliquot and is validated for Western Blotting (WB), Immunohistochemistry (IHC), and Immunocytochemistry/Immunofluorescence (ICC/IF), making it suitable for detection of full-length PARP1 (~116 kDa) as well as the cleaved fragments generated during programmed cell death. It reacts with human and mouse samples and is affinity-purified from rabbit serum (host: Rabbit; clonality: Polyclonal). According to the manufacturer's storage guidance shown for this family of Antibodies.com polyclonal PARP1 reagents, the vial is shipped at 4 °C and should be aliquoted and stored at -20 °C upon arrival, with freeze/thaw cycles avoided to preserve activity. The unconjugated format allows researchers to couple the antibody to their preferred fluorophore, biotin, or HRP depending on the downstream application, and the polyclonal nature provides recognition of multiple epitopes within the PARP1 protein, which is useful when probing both intact and proteolytically processed forms of the enzyme.