Anti-PARP1 Antibody

Raised in rabbit against the C-terminal region of poly(ADP-ribose) polymerase 1, this polyclonal reagent from Antibodies.com detects the ~116 kDa DNA-damage response enzyme in human and mouse samples. PARP1 catalyzes NAD+-dependent poly-ADP-ribosylation of nuclear acceptor proteins, drives base excision repair of single- and double-strand DNA breaks, and is a well-characterized substrate of executioner caspases, where cleavage at Asp214 produces 89 kDa and 24 kDa fragments that serve as a biochemical hallmark of apoptosis.

The unconjugated antibody is supplied as 100 µl of liquid antiserum suitable for western blot, immunohistochemistry, and immunofluorescence workflows. Researchers commonly apply it to study genotoxic stress, chromatin-associated signaling, and programmed cell death in cancer and neurodegeneration models. For long-term storage, aliquot the reagent to limit freeze-thaw cycles and keep at -20°C; protect from light and avoid prolonged exposure to room temperature to preserve binding activity.