Anti-Pancreatic Amylase Antibody

For western blot and immunohistochemistry workflows on human samples, this polyclonal reagent from Antibodies.com targets Pancreatic Amylase, the calcium-dependent starch-hydrolyzing enzyme secreted by acinar cells of the exocrine pancreas. Each vial delivers 100 µl of unconjugated rabbit antiserum, supporting multiple membrane and section staining experiments.

Probing amylase expression in tissue lysates or fixed sections aids research into acute and chronic pancreatitis, cystic fibrosis-related pancreatic insufficiency, exocrine pancreatic dysfunction, and the differentiation between pancreatic and salivary isoenzyme sources in digestive enzymology studies. The polyclonal host response recognizes multiple epitopes on the amylase molecule, helping when working with denatured protein on western blots or with native antigen preserved in formalin-fixed paraffin-embedded sections.

For best stability, store the reagent at -20°C in single-use aliquots to limit freeze-thaw cycles and preserve binding activity over the shelf life of the stock. Applications include western blot for protein-level confirmation in cell or tissue lysates and immunohistochemistry for spatial mapping of amylase expression across exocrine pancreatic tissue, ductal regions, and islet-adjacent acinar compartments.