Anti-PAK2 Antibody

A polyclonal reagent from Antibodies.com raised in rabbit against the p21-activated kinase 2 (PAK2) target, supplied as an unconjugated 100 µl aliquot for western blot and immunohistochemistry workflows. PAK2 is a widely expressed serine/threonine kinase that operates downstream of the small GTPases RAC1 and CDC42, relaying cytoskeletal and proliferative cues through phosphorylation of substrates including MEK1, RAF1, and MLC2.

The antibody recognizes human, mouse, and rat PAK2 in research settings, with the polyclonal format providing coverage of multiple epitopes on the target kinase. In western blot, PAK2 migrates near 58 kDa, enabling clear identification in lysates from cultured cell lines and primary tissue. For immunohistochemistry on paraffin-embedded sections, standard antigen retrieval methods yield cytoplasmic staining in cells exhibiting active PAK2 signaling, while chromogenic detection offers clear visualization of expression patterns.

Storage at refrigerated temperatures preserves reagent activity across multiple freeze-thaw cycles when small working aliquots are prepared. Researchers examining cytoskeletal remodeling, growth factor signaling, or apoptotic pathways can pair this reagent with appropriate loading controls to characterize PAK2 activation states across experimental model systems.