Anti-PAK1 (phospho T212) Antibody

Phospho-specific rabbit polyclonal reagent raised against p21-activated kinase 1 (PAK1) phosphorylated at threonine 212, supplied by Antibodies.com as a 50 µl unconjugated IgG preparation. The antibody selectively recognises the activated form of the kinase, an autophosphorylation mark on the activation loop that correlates with downstream cytoskeletal and MAPK-pathway signalling.

Recommended for Western blot analysis of human cell and tissue lysates, the reagent is provided at 50 µl per vial and can be combined with standard HRP- or fluorophore-conjugated secondary antibodies for chemiluminescent or fluorescent detection of active PAK1 in signalling studies, migration assays, and cancer research workflows investigating kinase-dependent growth and invasion pathways.

Because the reagent targets the phosphorylated T212 epitope rather than total PAK1, it is suited to time-course and stimulation experiments in which researchers compare basal versus agonist-induced kinase activation, including serum- or growth-factor-stimulated cell culture models. The polyclonal IgG host format offers consistent lot-to-lot behaviour for laboratory work probing Rac1/CDC42-driven PAK signalling in human samples.