Anti-PAK1 (phospho T212) Antibody

This phospho-specific polyclonal reagent from Antibodies.com targets PAK1 (p21-activated kinase 1) only when phosphorylated on threonine 212, an activation-site modification linked to growth-factor and integrin signaling cascades. Raised in rabbit and affinity-purified as an IgG preparation, the antibody is supplied unconjugated in a 200 µl volume, ready for direct incubation with standard Western blot workflows on human-derived lysates. Because the epitope includes the phosphate group at T212, the reagent discriminates catalytically engaged PAK1 from the inactive pool, which is why it is frequently used to track pathway activation downstream of Rac1/Cdc42, IGF-1, EGF, and integrin engagement in published studies of cytoskeletal remodeling, cell migration, and oncogenic transformation.

For Western blot, the antibody is typically applied to nitrocellulose or PVDF membranes following SDS-PAGE separation of whole-cell lysates prepared under phosphatase-inhibitor conditions to preserve the phospho-epitope. Store the vial at -20 °C in single-use aliquots to limit repeated freeze-thaw cycles, and protect from light. The host-species IgG is compatible with standard anti-rabbit HRP or fluorescent secondary reagents. Researchers studying breast cancer, melanoma, and other PAK1-driven tumour models commonly deploy this reagent alongside total-PAK1 loading controls to normalise phosphorylation signals across treatment conditions, and the human reactivity profile makes it a useful tool for confirmatory follow-up to phosphoproteomic screening data.