Anti-PAK1 / PAK2 / PAK3 (Ab-423 / 402 / 421) Antibody

This rabbit polyclonal reagent from Antibodies.com is supplied as an unconjugated, affinity-purified preparation at 1.0 mg/mL in a 50 µl volume, formulated in PBS (pH 7.4) containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol as a cryoprotectant suitable for standard laboratory storage at –20 °C. The antibody was raised in rabbit against a synthetic peptide corresponding to the region around residues 421–425 / 400–404 / 419–423 (R-S-T-M-V) of human PAK1 / PAK2 / PAK3, and was subsequently purified by affinity chromatography using the epitope-specific peptide, ensuring selective recognition of total endogenous PAK1, PAK2, and PAK3 protein across human, mouse, and rat samples. It is validated for western blotting (WB), immunohistochemistry (IHC), and immunocytochemistry / immunofluorescence (ICC/IF), making it a versatile tool for studying p21-activated kinase signalling in cell lysates, tissue sections, and cultured cells. As a polyclonal reagent, the antiserum offers broad epitope coverage of the conserved phosphorylated and unphosphorylated regions surrounding the targeted sites, which is particularly useful when probing activation states of the PAK family. Supplied unconjugated, the reagent can be directly labelled or paired with secondary antibodies of the researcher's choice, and the 50 µl pack size is intended for research use only, not for diagnostic or therapeutic applications.