Anti-PAK1 / PAK2 / PAK3 (Ab-423 / 402 / 421) Antibody

This rabbit polyclonal antibody from Antibodies.com recognizes the activated forms of the p21-activated kinase (PAK) family at their conserved autophosphorylation sites — threonine 423 in PAK1, threonine 402 in PAK2, and threonine 421 in PAK3. Because these residues lie within the kinase activation loop, the reagent is best suited for tracking active PAK signaling rather than total PAK expression.

Validated applications include Western blot, immunohistochemistry, and immunofluorescence. The antibody is unconjugated and is supplied as 100 µl of liquid stock. Reactivity has been confirmed in Human, Mouse, and Rat samples, supporting translational work across the most commonly used mammalian model systems.

PAK1, PAK2, and PAK3 carry overlapping and isoform-specific roles in cytoskeletal remodeling, cell migration, survival signaling, and neuronal development. Researchers use this reagent to study Rac/Cdc42-driven kinase activation in cancer cell invasion, immune cell chemotaxis, growth factor responses, and synaptic plasticity. For Western blot sample preparation, fresh or snap-frozen lysates prepared with phosphatase inhibitors help preserve the phospho-epitope before SDS-PAGE separation.

For handling, store at -20 °C in working aliquots to limit freeze-thaw cycles, and the unconjugated format allows flexible pairing with HRP- or fluorophore-conjugated secondary antibodies for chromogenic or immunofluorescence readouts.