Anti-PAK1 (Ab-212) Antibody

From the catalogue of Antibodies.com, this affinity-purified polyclonal reagent is raised in rabbit against the serine/threonine kinase PAK1 and is offered in a 50 µl pack at a stated protein concentration of 1.0 mg/ml. The immunogen corresponds to a short synthetic peptide spanning amino acids 210–214 (P-V-T-P-T) of human PAK1, allowing the antibody to detect endogenous levels of total PAK1 protein in human, mouse and rat samples. The unconjugated antibody has been validated for Western blotting, immunohistochemistry on paraffin-embedded tissue and immunocytochemistry/immunofluorescence staining, with representative images on the supplier page showing bands in 293 and 3T3 cell lysates together with staining of human breast carcinoma sections. The formulation is supplied in phosphate-buffered saline (pH 7.4, without Mg²⁺ and Ca²⁺) containing 150 mM sodium chloride, 0.02% sodium azide and 50% glycerol; the product is shipped refrigerated at 4 °C and should be aliquoted and stored at –20 °C, avoiding repeated freeze/thaw cycles. Antibodies were produced by immunizing rabbits with the peptide–KLH conjugate and purified by affinity chromatography against the epitope-specific peptide. Intended strictly for research use, this reagent offers a reliable option for laboratories studying PAK1 signalling in cell biology, cancer research and related fields.