Anti-PAK1 (Ab-204) Antibody

This rabbit polyclonal reagent from Antibodies.com targets PAK1 (p21-activated kinase 1), a serine/threonine kinase that acts downstream of the Rho-family GTPases Rac1 and Cdc42 and plays a central role in cytoskeletal reorganization, focal adhesion turnover, cell migration, and MAPK cascade signaling. The antibody was raised against a synthetic peptide surrounding residue 204 of human PAK1, a region that includes an autophosphorylation site frequently used as a read-out of kinase activation.

Supplied as 50 µl of unconjugated antiserum, the antibody is validated for Western blot and immunohistochemistry on human samples. In WB workflows it produces a clean band at the expected ~68 kDa molecular weight, while in IHC it produces cytoplasmic and membranous staining consistent with PAK1 distribution in epithelial and neural tissues. Researchers studying cytoskeletal remodeling, neuronal pathfinding, breast cancer cell invasion, and RAF/MEK/ERK pathway crosstalk routinely deploy anti-PAK1 antibodies to monitor total kinase levels and to compare expression between normal and transformed specimens.

The polyclonal format offers broader epitope coverage than a monoclonal reagent, which is helpful when probing PAK1 isoforms or truncation variants. Because the antibody is unconjugated, it can be paired with HRP, biotin, or Alexa Fluor secondary reagents to suit chromogenic, fluorescence, or tyramide-based detection platforms. For best results, store small aliquots frozen at -20 °C, avoid repeated freeze-thaw cycles, and centrifuge the vial briefly before pipetting to recover the full 50 µl volume.