Anti-PAK1 (Ab-204) Antibody

This reagent from Antibodies.com detects PAK1 (p21-activated kinase 1) at the Ab-204 epitope within the protein, a target used to monitor PAK1 expression and modification status in cell signaling studies. PAK1 sits downstream of the Rho-family GTPases Cdc42 and Rac1 and couples those inputs to cytoskeletal reorganization, focal-adhesion turnover, MAPK cascade activation, and survival pathways such as AKT and NF-κB. Investigators in cancer biology, neuroscience, vascular biology, and immunology rely on robust detection of this kinase to monitor pathway engagement in cell lines, primary cultures, and tissue specimens.

Validated for Western blot (WB) and immunohistochemistry (IHC) on human samples, the antibody is supplied unconjugated at 100 µL per vial, allowing flexible pairing with HRP-, biotin-, or fluorophore-conjugated secondary reagents of the researcher's choice. The polyclonal format increases tolerance for minor epitope variations caused by adjacent post-translational modifications and provides stronger signal in IHC where epitope accessibility can be limiting. Typical workflows include probing lysates from cultured cells with serum- or growth-factor-induced PAK1 activation as a positive control, and staining formalin-fixed paraffin-embedded tumor sections to assess PAK1 activity in situ.

Storage at 4 °C for short-term use, with aliquoting and frozen long-term storage, preserves activity across repeated freeze-thaw events. Host species is rabbit, clonality is polyclonal, and the validated target-species reactivity is human, making this antibody suitable for translational studies on patient-derived samples and standard cancer cell line panels.