Anti-PAK1 / 2 (Ab-199) Antibody

Offered by Antibodies.com as a research-grade primary antibody, this rabbit polyclonal reagent specifically recognizes the PAK1/2 kinases (p21-activated kinases 1 and 2) at a region surrounding the serine 199 phosphorylation site, detecting total endogenous levels of PAK1/2 protein irrespective of phosphorylation state. The immunogen is a synthetic non-phosphopeptide derived from human PAK1/2, corresponding to residues flanking the T-K-S(p)-V-Y motif, and the antibody was affinity-purified from rabbit antiserum by epitope-specific affinity chromatography to maximize target selectivity. The host species is rabbit (rabbit IgG isotype), the clonality is polyclonal, and the conjugate is unconjugated, allowing flexibility for downstream detection labeling in user-optimized assays. Validated applications include Western blot (WB) and immunohistochemistry (IHC), and the antibody has been confirmed to react with Human, Mouse, and Rat samples, supporting cross-species studies in cell biology and signal-transduction research. The product is supplied at a concentration of 1.0 mg/mL in a 50 µL volume (a 100 µL size is also offered), formulated in phosphate-buffered saline without Mg²⁺ and Ca²⁺ (pH 7.4), 150 mM NaCl, 0.02% sodium azide, and 50% glycerol. For handling, the vial is shipped at 4 °C; upon receipt it should be aliquoted and stored at −20 °C, with repeated freeze/thaw cycles avoided to preserve activity. Intended strictly for research use, this Antibodies.com reagent is a reliable tool for investigating PAK1/2-driven pathways involved in cytoskeletal remodeling, cell migration, proliferation, and related signaling cascades.