Anti-PAICS Antibody

Raised in rabbit against the bifunctional de novo purine biosynthesis enzyme PAICS (phosphoribosylaminoimidazole carboxylase / phosphoribosylaminoimidazole succinocarboxamide synthetase), this unconjugated polyclonal primary antibody from Antibodies.com is supplied as 100 µL of antiserum and validated for Western blot and immunofluorescence. It cross-reacts with human, mouse, and rat samples, supporting comparative studies of nucleotide metabolism across cell and animal model systems.

PAICS catalyzes steps 6 and 7 of the purine biosynthesis pathway and is frequently upregulated in proliferating cells and in several cancer types, where increased flux supports the nucleic acid demand of rapid division. The polyclonal format recognizes multiple epitopes on the target, which is useful when probing full-length PAICS expression, isoform patterns, and changes induced by pathway inhibitors or metabolic perturbation experiments. Researchers studying tumor metabolism, cell-cycle progression, and antifolate drug response commonly use this reagent to monitor endogenous PAICS protein levels.

Typical workflows include immunoblotting of whole-cell or tissue lysates to quantify PAICS band intensity against loading controls, paired with staining of fixed cultured cells to examine subcellular localization. The unconjugated format allows the user to pair the primary reagent with HRP- or fluorophore-conjugated secondary antibodies of their choice, keeping detection chemistry flexible across imaging, blotting, and downstream quantification platforms.