Anti-PAF1 Antibody

RNA polymerase II-associated factor 1 (PAF1) is a nuclear subunit of the PAF1C transcription complex that associates with RNA polymerase II to support transcription elongation, histone H2B monoubiquitination, and 3′ end processing of polyadenylated mRNAs. This Antibodies.com reagent is a rabbit polyclonal affinity reagent raised against the human protein, unconjugated and supplied in a 200 µl vial of liquid buffer.

Intended for research use only, the antibody reacts with the human target and is validated for western blot detection of endogenous PAF1 in lysates prepared from cultured human cells or tissue samples under standard RIPA conditions. Aliquot on first use and avoid repeated freeze-thaw cycles to preserve polyclonal IgG activity across multiple immunoblotting experiments.

For WB workflows, dilute in blocking buffer according to the secondary antibody system in use; an HRP-conjugated anti-rabbit secondary supports a clear band at the predicted molecular weight region corresponding to PAF1 in human nuclear and whole-cell extracts.