Anti-p95 / NBS1 (Ab-343) Antibody

This rabbit polyclonal reagent from Antibodies.com targets the phosphorylated form of p95/NBS1 (NBN/Nibrin) at serine 343, a residue directly modified by ATM kinase during the cellular DNA double-strand break response. The unconjugated antibody is supplied as 100 µl of liquid and is validated for Western blot and immunohistochemistry workflows on human samples, where it enables visualization of activated NBS1 within pathways governing genomic stability, checkpoint control, and homologous recombination repair.

Raised against the Ab-343 phospho-epitope, the polyclonal preparation offers broader epitope coverage useful when surveying phosphorylation status across denatured lysates (Western blot) and fixed tissue sections (immunohistochemistry). Researchers studying Nijmegen breakage syndrome, ATM signaling cascades, radiation-induced DNA damage, or cell cycle checkpoint regulation rely on phospho-Ser343 NBS1 detection as a marker of MRN complex activation.

Store at -20 °C in working aliquots to limit freeze-thaw cycles; the unconjugated format allows flexible pairing with HRP-, fluorophore-, or biotin-conjugated secondary reagents. Host species, clonality, and target specificity are noted on the vial label and accompanying datasheet.