Anti-P90RSK (phospho T348) Antibody - Identical to Abcam (ab194743)

p90RSK (also known as RSK1, RPS6KA1, and MAPKAP-K1a) is a serine/threonine kinase that sits downstream of the RAS/MAPK cascade, and phosphorylation at threonine 348 within its activation loop, driven by ERK1/2, is the modification required for full catalytic output. Antibodies.com raises this rabbit polyclonal IgG against a synthetic phosphopeptide spanning the S-R-T(p)-P-R sequence around T348, so the reagent selectively detects endogenous p90RSK only when that residue is phosphorylated.

The unconjugated antibody is supplied at 1.0 mg/mL in a 200 µl vial of PBS pH 7.4 with 150 mM NaCl, 0.02% sodium azide, and 50% glycerol. Affinity purification against the epitope-specific phosphopeptide, with subtraction of non-phospho-specific IgG, leaves a reagent that recognizes the active kinase. The predicted band is 90 kDa, and the antibody has been validated on HeLa lysates with and without UV induction, on paraffin-embedded human breast carcinoma tissue, and on methanol-fixed HeLa cells. Recommended starting dilutions are 1:500–1:1000 for western blot, 1:50–1:100 for immunohistochemistry, and 1:100–1:200 for immunofluorescence on human, mouse, and rat samples.

The vial is shipped at 4°C; aliquot on arrival and store at -20°C, and minimize freeze-thaw cycles to preserve IgG activity. Researchers in cell signaling, cancer biology, and neuroscience use this reagent as a downstream readout of ERK pathway engagement, and the product has been benchmarked as functionally identical to Abcam ab194743. For research use only.