Anti-P70S6K1 (phospho S424) Antibody

This rabbit polyclonal phospho-specific antibody recognizes P70S6K1 only when its serine 424 residue carries a phosphate group, allowing researchers to monitor kinase activation-state dynamics independently of total protein abundance in cell lysates. From Antibodies.com, the reagent ships as an unconjugated IgG preparation at 50 µl per vial, ready to pair with HRP- or fluorophore-conjugated secondary antibodies for chemiluminescent or fluorescent Western blot detection. Confirmed reactivity is with human P70S6K1, and the antibody is validated specifically for Western blot workflows on human samples.

Applications of interest include dissecting mTORC1 pathway output, insulin and growth factor signaling analysis, translation initiation studies, and any research where S424 phosphorylation is the readout rather than total kinase level. The polyclonal format provides broader epitope coverage around the phospho-S424 site, which can be advantageous when probing different human cell lines and primary tissue samples. Recommended handling is to aliquot upon arrival and store frozen to preserve phospho-epitope integrity; minimize freeze-thaw cycles by working from a small active aliquot, and protect from light during manipulation.