Anti-p70 S6K (phospho-T421) Antibody

P70 S6 kinase (RPS6KB1) is a serine/threonine kinase downstream of mTOR complex 1 that drives protein synthesis, cell growth, and proliferation. Phosphorylation at threonine 421 within its autoinhibitory linker region is one of the activating events in this pathway, and detecting this modification is a standard read-out of mTORC1 activity in studies of insulin signaling, cancer cell proliferation, muscle hypertrophy, and neurodegeneration.

This unconjugated rabbit polyclonal antibody from Antibodies.com specifically recognizes p70 S6K when phosphorylated at T421 and has been validated for Western blot and immunohistochemistry in human, mouse, and rat samples. The reagent is supplied as a 100 µL volume at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol, ready to pair with standard HRP- or fluorophore-conjugated secondary reagents for chemiluminescent or fluorescent detection workflows.