Anti-p70 S6K (phospho-S424) Antibody

This rabbit polyclonal reagent from Antibodies.com is raised against p70 S6 kinase phosphorylated at serine 424, a regulatory autophosphorylation site within the C-terminal domain of the 70 kDa ribosomal protein S6 kinase. It is supplied at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol, and is packaged as a 100 µl vial of unconjugated polyclonal antiserum validated for western blot and immunohistochemistry workflows. Documented reactivity covers human, mouse, and rat samples, supporting its use across standard mammalian systems in signal transduction research on the mTOR and PI3K/AKT pathways.

For routine use, dilute in blocking buffer according to your western blot or immunostaining protocol and store the vial at -20 °C for long-term stability; aliquoting on first use helps limit freeze-thaw damage to the immunoglobulin preparation. The phospho-S424 epitope serves as a marker of activated p70 S6K and is frequently employed in studies of translational control, cell growth, and metabolic regulation. Researchers should pair it with appropriate positive controls, such as serum- or growth-factor-stimulated cell lysates, and where needed a pan-p70 S6K antibody to compare phosphorylated versus total kinase levels.