Anti-p70 S6K (phospho-S418) Antibody

This polyclonal phospho-specific reagent from Antibodies.com selectively detects the activated form of p70 S6 kinase (p70 S6K) when phosphorylated at serine 418, a regulatory site within the kinase activation loop whose modification is driven by mTORC1 signaling. Raised in rabbit, the antibody is supplied at 1 mg/ml in PBS with 0.1% sodium azide and 50% glycerol, packaged as a 100 µl vial in unconjugated format suited to either western blot (WB) or immunohistochemistry (IHC) workflows.

Phosphorylation at S418 is a key step that primes p70 S6K for full catalytic activity and downstream phosphorylation of ribosomal protein S6, translation initiation factors, and other substrates linked to protein synthesis and cell growth control. Researchers investigating mTOR pathway activation, nutrient sensing, insulin signaling, muscle hypertrophy, and tumor cell proliferation use this reagent to monitor the activation state of p70 S6K across Human, Mouse, and Rat samples. Validated reactivity with these species enables comparative work in cell lines, primary tissues, and animal models.

The polyclonal host provides recognition of multiple epitopes surrounding the phospho-S418 site, supporting reliable detection in lysates prepared from stimulated versus resting conditions. Pairing this reagent with corresponding total p70 S6K antibody controls is recommended to normalize for expression differences and to interpret changes in pathway activation accurately. For storage, refrigerate and protect from light to preserve antibody activity through the product shelf life.