Anti-p70 S6 Kinase (Ab-424) Antibody

Produced by Antibodies.com, this rabbit polyclonal reagent targets a phosphorylated epitope surrounding residues 422–426 (P-V-S-P-V) of human p70 S6 Kinase, enabling detection of total endogenous RPS6KB1 protein. Supplied as a 50 µL aliquot at 1.0 mg/mL in phosphate-buffered saline (pH 7.4) containing 150 mM NaCl, 0.02% sodium azide, and 50% glycerol, the unconjugated IgG is validated for Western blotting (1:500–1:1000), immunohistochemistry (1:50–1:100), and immunofluorescence (1:100–1:200), with reactivity confirmed against Human, Mouse, and Rat samples and predicted bands at approximately 70 and 85 kDa. The antibody is purified by epitope-specific peptide affinity chromatography following immunization with a KLH-conjugated synthetic peptide, then shipped at 4 °C for short-term transit; for long-term use it should be aliquoted and stored at −20 °C with freeze/thaw cycles avoided to preserve performance. Intended for research use only and not for diagnostic or therapeutic applications, this lot offers a reliable tool for laboratories studying mTOR pathway signaling, ribosomal protein S6 phosphorylation, and downstream translational control in cell and tissue models.