Anti-p70 S6 Kinase (Ab-424) Antibody

This rabbit polyclonal antibody from Antibodies.com specifically recognizes p70 S6 Kinase at the Ab-424 epitope, a threonine residue within the kinase activation loop that is regulated by mTOR-dependent signaling. The unconjugated reagent is supplied in 100 µl volumes and is validated for Western blot, immunohistochemistry, and immunofluorescence workflows using human, mouse, and rat samples.

p70 S6 Kinase (also known as RPS6KB1 or S6K1) operates as a downstream effector of the mTOR pathway, where it controls protein synthesis, cell growth, and metabolic responses through phosphorylation of ribosomal protein S6 and additional substrates. Detection of the Ab-424 phospho-epitope offers a useful readout of mTORC1 pathway activation in research studies of signaling dynamics, nutrient sensing, and translational control. The polyclonal format provides broader epitope recognition than monoclonal alternatives, while the unconjugated presentation allows flexible pairing with secondary reagents for fluorescent, biotin, or enzyme labeling.

Typical uses include Western blot analysis of whole-cell lysates to monitor kinase phosphorylation status, immunohistochemistry on fixed tissue sections to localize active kinase distribution, and immunofluorescence staining for subcellular localization studies. Cross-reactivity is confirmed for human, mouse, and rat specimens, supporting comparative work across standard cell culture and animal tissue models.