Anti-p70 S6 Kinase (Ab-421) Antibody

Rabbit polyclonal antibodies that selectively recognize the phosphorylated threonine 421 site on p70S6K are valuable tools for tracking mTOR pathway activation in cell signaling research. This unconjugated reagent from Antibodies.com is raised against the Ab-421 epitope and supplied as 50 µl of antiserum in a vial format, ready for direct use or further purification.

Validated for Western blot and immunohistochemistry, the antibody is reactive with Human, Mouse, and Rat samples, supporting studies across cell line lysates, primary cultures, and rodent tissue sections. p70 S6 Kinase is a 70 kDa serine/threonine kinase that acts downstream of mTOR to drive protein synthesis and cell growth, and phosphorylation at T421 reflects pathway activity stimulated by growth factors, nutrients, or cellular stress cues.

Researchers commonly use the Ab-421 reagent to compare basal versus stimulated signaling states, monitor kinase inhibitor effects on the mTOR axis, and profile pathway activation in tumor versus normal tissue. The antibody is intended for research use only and should be aliquoted to limit repeated freeze-thaw cycles of the stock vial.