Anti-p70 S6 Kinase (Ab-389) Antibody

This rabbit polyclonal reagent from Antibodies.com is raised against p70 S6 Kinase at the Ab-389 epitope, using a synthetic peptide sequence spanning residues 387–391 (G-F-T-Y-V) of the human protein. Supplied as an unconjugated, affinity-purified preparation at 1.0 mg/mL in a 50 µl volume of phosphate-buffered saline (pH 7.4, without Mg²⁺ and Ca²⁺) containing 150 mM NaCl, 0.02% sodium azide and 50% glycerol, the antibody is designed to detect the endogenous level of total p70 S6 Kinase across human, mouse and rat samples. It is validated for Western blotting (WB) and immunocytochemistry/immunofluorescence (ICC/IF), making it well suited to studies of mTOR pathway signalling where total RPS6KB1/p70 levels need to be monitored alongside phospho-specific readouts. Production is carried out by immunising rabbits with the peptide–KLH conjugate, followed by affinity-chromatography purification against the epitope-specific peptide, which gives the polyclonal reagent broad recognition of native target epitopes. The product is shipped on cold packs at 4 °C from St. Louis, MO, with a typical lead time of 6–9 business days; upon arrival it should be aliquoted and stored at –20 °C, avoiding repeated freeze/thaw cycles to preserve binding activity. This reagent is intended for research use only and is not for diagnostic or therapeutic applications.