Anti-p70 S6 kinase α Antibody

This rabbit polyclonal primary antibody from Antibodies.com targets the α isoform of p70 S6 kinase, a serine/threonine kinase that sits downstream of mTOR complex 1 (mTORC1) and phosphorylates ribosomal protein S6 to drive translation initiation and cell growth. Researchers rely on it to monitor mTOR pathway activation in signalling, metabolism, and cancer studies where phosphorylated and total p70 levels are routinely compared.

Supplied as 100 µl of unconjugated antisera, the reagent is validated for western blot, immunohistochemistry, immunofluorescence, and ELISA workflows. It recognises the kinase in human, mouse, and rat samples, making it practical across standard laboratory models. Polyclonal reactivity broadens epitope coverage, which is helpful when probing conserved kinase domains or mapping isoform-specific phosphorylation sites such as Thr389 and Thr229.

For western blot, working dilutions of roughly 1:500 to 1:2000 are common depending on expression level; for IHC and IF on fixed cells or cryosections, 1:100 to 1:500 is frequently used. Aliquot upon arrival to limit freeze-thaw cycles, store refrigerated, and protect from light. Intended for research use only, not for diagnostic or therapeutic applications.