Anti-p70 S6 kinase α Antibody

Manufactured by Antibodies.com, this affinity-purified polyclonal reagent is raised in rabbit against p70 S6 kinase α (also known as RPS6KB1, p70 S6K-alpha, S6K1, or STK14A), a 70 kDa serine/threonine kinase that plays a central role in the mTOR signaling pathway and the regulation of protein synthesis and cell growth. The antibody is supplied as an unconjugated IgG polyclonal at a concentration of 1 mg/ml in a 50 µl vial, provided in liquid form in PBS containing 50% glycerol, 0.5% BSA, and 0.02% sodium azide. It is intended for research use only and is not approved for diagnostic or therapeutic applications. Validated applications include Western blotting (WB), immunohistochemistry (IHC), immunofluorescence (IF), and ELISA, and the reagent has been confirmed to react with human, mouse, and rat samples. The immunogen is a synthesized peptide derived from the human p70 S6 kinase α sequence, and the antibody specifically detects endogenous levels of total p70 S6 kinase α protein. For optimal stability, the product should be stored at −20 °C, where it remains stable for up to one year, allowing continuous use over extended experimental timelines. This reagent is well suited for laboratories investigating translational control, insulin and growth-factor signaling, the PI3K/AKT/mTOR axis, and downstream phosphorylation events that regulate ribosomal protein S6 and protein synthesis. As with all affinity-purified polyclonal preparations, batch-to-batch consistency should be verified by the end user, and appropriate secondary antibodies should be selected according to the chosen detection method and host species.