Anti-p65 Antibody

The p65 subunit, also known as RelA, is the principal transactivating component of the NF-κB transcription factor complex and a cornerstone of inflammatory signaling, innate immunity, and cell-survival research. This rabbit polyclonal antibody from Antibodies.com specifically detects endogenous p65 protein in human, mouse, and rat samples, and is validated for western blot, immunohistochemistry, and immunofluorescence workflows.

Under resting conditions, p65 is held in the cytoplasm by IκBα inhibitory proteins. Pathway stimulation through TNF-α, IL-1β, LPS, or oxidative stress triggers IκBα phosphorylation and proteasomal degradation, releasing p65 to translocate into the nucleus and activate transcription of target genes including cytokines, chemokines, and anti-apoptotic factors such as Bcl-2 and XIAP. Researchers use this reagent to monitor NF-κB activation kinetics, characterize IKK inhibition by small molecules, evaluate inflammatory status in disease models, or study tumor-promoting inflammation in cancer biology.

The reagent is supplied as 50 µl of unconjugated rabbit antiserum, allowing flexible pairing with user-selected HRP, biotin, or fluorophore secondary reagents for chromogenic, fluorescent, or multiplexed detection. Aliquoting upon arrival is recommended to limit repeated freeze-thaw cycles, with short-term storage at 4°C and long-term storage at -20°C or colder for retained activity across many experiments.