Anti-p63 Antibody

The p63 transcription factor belongs to the p53 family of tumor suppressors and governs epithelial development, stem cell renewal, and squamous-lineage commitment. This polyclonal reagent from Antibodies.com targets epitopes shared across the major p63 isoforms, including the transactivating (TAp63) and dominant-negative (ΔNp63) variants that mark basal keratinocytes and respiratory epithelium.

Raised in rabbit and supplied as an unconjugated 100 µL vial of affinity-purified IgG, the antibody supports Western blot detection of endogenous p63 in lysates prepared from human, mouse, and rat tissues. ELISA compatibility is also validated, making the reagent useful for quantifying recombinant p63 expression in cell-line screens or for probing interaction partners of the transcription factor.

Working applications include verifying p63 status in keratinocyte differentiation models, characterizing squamous cell carcinoma biopsy lysates, and confirming basal-cell identity in organoid cultures. For best results, lyse samples in SDS buffer, separate by electrophoresis, transfer to PVDF or nitrocellulose, and detect with chemiluminescence. Store refrigerated; aliquot to avoid repeated freeze-thaw cycles that reduce polyclonal titer over time.