Anti-p53 (V31) Antibody

Rabbit polyclonal antibodies targeting the p53 tumor suppressor protein provide a versatile reagent for cancer biology and apoptosis research. This unconjugated primary antibody from Antibodies.com is supplied at 1 mg/ml in phosphate buffered saline (PBS) containing 0.05% sodium azide at approximately pH 7.2, packaged in a 50 µl vial suitable for multiple experimental runs. Validated applications include Western blot, immunohistochemistry, and immunofluorescence workflows, with reactivity confirmed against human, mouse, and rat samples. Researchers investigating DNA damage response, cell cycle checkpoint control, and oncogenic transformation can use this reagent to detect wild-type and mutant p53 in cell lysates and formalin-fixed tissue sections. For routine use, refrigerate at 4 °C; for extended storage, freeze at -20 °C in single-use aliquots to limit freeze-thaw cycles and preserve antibody activity. The polyclonal host response directed against the V31 epitope region allows recognition of multiple antigenic sites, which can be advantageous when probing denatured proteins or fixed specimens where a single monoclonal epitope may be inaccessible.