Anti-p53 (V31) Antibody

Raised in rabbit against the p53 tumor suppressor, this unconjugated polyclonal primary antibody from Antibodies.com is supplied at 1 mg/ml in phosphate buffered saline with 0.05% sodium azide at approximately pH 7.2. The reagent is validated for western blot, immunohistochemistry, and immunofluorescence workflows, with reactivity confirmed against human, mouse, and rat samples. Each vial contains 100 µl of working stock, providing enough material for routine detection of endogenous p53 in cell lysates and fixed tissue sections used to interrogate DNA damage response, apoptosis, and oncogenic signaling pathways. The polyclonal format offers recognition of multiple epitopes on the TP53 protein, which can be advantageous when studying mutant or post-translationally modified p53 variants compared with monoclonal alternatives. Store refrigerated and protect from light to preserve activity, aliquot upon first use to limit freeze-thaw cycles, and pair with HRP- or fluorophore-conjugated secondary antibodies for chromogenic or fluorescence-based readout. Intended for research use only in vitro.