Anti-p53 (R375) Antibody

This rabbit polyclonal antibody from Antibodies.com recognizes the p53 tumor suppressor (TP53) at an epitope centered around residue R375 in the C-terminal regulatory region of the human protein. The reagent is supplied as 100 µl of unconjugated liquid at 1 mg/ml in phosphate buffered saline (PBS) containing 0.05% sodium azide at approximately pH 7.2, enabling direct labeling or pairing with secondary antibody detection reagents.

Validated for Western blot (WB), immunohistochemistry (IHC), and immunofluorescence (IF), the antibody supports detection of endogenous p53 in cell lysates, fixed tissue sections, and cultured cells. Reactivity has been confirmed against human, mouse, and rat samples, making it useful for comparative studies of DNA damage response, apoptosis induction, cell cycle arrest, and tumor biology across standard laboratory models.

For handling, store the vial refrigerated and protect from light; aliquot the stock to limit repeated freeze-thaw cycles. Sodium azide is included as a preservative, so handle the product using standard laboratory safety precautions. Each vial is intended for research use only and is supplied in a liquid format that is ready for dilution into standard assay buffers.