Anti-p53 (M40) Antibody

Rabbit polyclonal antibody raised against the N-terminal epitope of p53, the tumor suppressor frequently mutated across human cancers. This unconjugated reagent is supplied as 50 µl at 1 mg/ml in phosphate buffered saline with 15 mM sodium azide at approximately pH 7.2, ready for direct use in Western blot workflows. The antibody recognizes endogenous p53 in human, mouse, and rat samples and is validated for immunoblotting of whole-cell lysates, where it yields a band near 53 kDa, the predicted molecular weight of full-length TP53.

Because the immunogen lies within an N-terminal region conserved across multiple species, this reagent supports cross-species studies of cellular stress responses, DNA damage signaling, and apoptosis research, and detects both wild-type and mutant forms of p53. The PBS-based formulation, with 15 mM sodium azide acting as a microbial preservative, enables immediate dilution into standard blocking buffers without prior buffer exchange. Sourced from Antibodies.com, the reagent serves as a research-use-only tool for laboratory investigation of p53 pathway biology, including regulation by MDM2, transactivation of downstream target genes such as p21, and p53's contribution to cellular senescence and tumor suppression models in cultured cells and tissue samples.