Anti-p53 (M40) Antibody

This rabbit polyclonal antibody from Antibodies.com targets the M40 epitope of human p53, the ~53 kDa tumor suppressor transcription factor encoded by the TP53 gene. Supplied as a 100 µl aliquot at 1 mg/ml in phosphate buffered saline containing 15 mM sodium azide (pH 7.2), the reagent is unconjugated and validated for Western blot detection of endogenous p53 in human, mouse, and rat samples. The M40 epitope maps within the N-terminal transactivation domain, allowing recognition of both wild-type p53 and many mutant variants commonly encountered in cancer cell line studies. Storage at -20 °C is recommended, with aliquoting on receipt to limit freeze-thaw exposure of the antibody; thawed material may be kept refrigerated for short-term use. Researchers apply this reagent to investigate DNA damage responses, apoptosis, cell cycle arrest, and oncogenic stress pathways in lysates from cultured cells and primary tissues.